Traumatic Shock. Vii. a Study of the Problem of the "lost Plasma" in Hemorrhagic, Tourniquet, and Burn Shock by the Use of Radioactive Iodo-plasma Protein.
نویسندگان
چکیده
In paper IV of this series (1), evidence was given that there was no significant loss of plasma into the tissues of the dog in untreated fatal shock from hemorrhage. The technic utilized involved the tagging of plasma proteins by radioactive sulfur or radioactive bromine. Before extending the observations to shock in which there is an area of local injury, such as occurs in tourniquet and burn shock, an attempt was made to reduce the errors inherent in the radioactive technic (1 to 4). These errors center in (1) the problem of obtaining the lowest possible halogen composition of the tagged protein in order to reduce denaturation of the protein to a minimum; (2) methods of determining the total intravascular plasma protein content of the tissues to be analyzed. This requires as accurate an estimate as can be made of the red cell fraction of the capillary blood. For this purpose, in addition to hemoglobin determinations of perhaps somewhat doubtful accuracy, estimates of the capillary hematocrit have been utilized. (3) The preparation of tissues for analysis so as to permit maximum recovery of the radioactive element. In preparing tissues containing radioactive bromo-protein for radioactivity measurements, an oxidative method of isolating the halogen was used. In the oxidation procedure, there was a possibility of loss of some bromine by volatilization in spite of the presence of AgNOa. Further, the excess bromide added as a carrier, together with the chloride also present, produced with silver nitrate a precipitate of sufficiently variable thickness and particle size to affect by self-absorption the accuracy of the radioactivity
منابع مشابه
Traumatic Shock: Iv. a Study of the Problem of the "lost Plasma" in Hemorrhagic Shock by the Use of Radioactive Plasma Protein.
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عنوان ژورنال:
- The Journal of clinical investigation
دوره 23 5 شماره
صفحات -
تاریخ انتشار 1944